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This protocol details a method for 5-ethynyl uridine labeling of nascent mitochondrial genome transcription coupled with immunofluorescence detection of mtDNA-binding proteins in cultured cells. The result is a fluorescent readout of localized mitochondrial gene transcription compatible with high-resolution microscopy.more » « lessFree, publicly-accessible full text available November 4, 2025
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Kakudji, Eve V; Lewis, Samantha C (, Current Biology)Eve Kakudji and Samantha Lewis discuss the structure and function of mitochondrial nucleoids - large nucleoprotein complexes containing mitochondrial DNA and the regulatory factors necessary for its packaging, replication, transcription, and repair.more » « less
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Mays, Alfred; Byars-Winston, Angela; Hinton, Antentor; Marshall, Andrea G.; Kirabo, Annet; August, Avery; Marlin, Bianca J.; Riggs, Blake; Tolbert, Blanton; Wanjalla, Celestine; et al (, Cell)
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