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Creators/Authors contains: "Lewis, Samantha C"

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  1. This protocol details a method for 5-ethynyl uridine labeling of nascent mitochondrial genome transcription coupled with immunofluorescence detection of mtDNA-binding proteins in cultured cells. The result is a fluorescent readout of localized mitochondrial gene transcription compatible with high-resolution microscopy. 
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    Free, publicly-accessible full text available November 4, 2025
  2. This protocol describes the metabolic labeling of mitochondrial translation in cultured cells (such as IMR90) using an amino acid L-Homopropargylglycine (L-HPG). 
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  3. Eve Kakudji and Samantha Lewis discuss the structure and function of mitochondrial nucleoids - large nucleoprotein complexes containing mitochondrial DNA and the regulatory factors necessary for its packaging, replication, transcription, and repair. 
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